GAPDH (6C5) 内参抗体 : sc-32233

BACKGROUND

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), also called uracil DNA glycosylase, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. While GAPDH has long been recognized as playing an integral role in glycolysis, additional functions of GAPDH include acting as a uricil DNA glycosylase, activating transcription, binding RNA and involvement in nuclear RNA export, DNA replication and DNA repair. Expression of GAPDH is upregulated in liver, lung and prostate cancers. GAPDH translocates to the nucleus during apoptosis. GAPDH complexes with neuronal proteins implicated in human neuro-degenerative disorders including the β-Amyloid precursor, Huntingtin and other triplet repeat neuronal disorder proteins.

CHROMOSOMAL LOCATION Genetic locus:

GAPDH (human) mapping to 12p13.31; Gapdh (mouse) mapping to 6 F3.

SOURCE

GAPDH (6C5) is a mouse monoclonal antibody raised against GAPDH purified from rabbit muscle.

PRODUCT

Each vial contains 100 µg IgG1 in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

APPLICATIONS

GAPDH (6C5) is recommended for detection of GAPDH of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)] and immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500). Suitable for use as control antibody for GAPDH siRNA (h): sc-35448, GAPDH siRNA (m): sc-35449, GAPDH shRNA Plasmid (h): sc-35448-SH, GAPDH shRNA Plasmid (m): sc-35449-SH, GAPDH shRNA (h) Lentiviral Particles: sc-35448-V and GAPDH shRNA (m) Lentiviral Particles: sc-35449-V. Molecular Weight of GAPDH: 37 kDa. Positive Controls: GAPDH (h): 293T Lysate: sc-159909, Jurkat whole cell lysate: sc-2204 or KNRK whole cell lysate: sc-2214.

STORAGE

Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. PROTOCOLS

See our web site at www.scbt.com or our catalog for detailed protocols and support products.

RESEARCH USE

For research use only, not for use in diagnostic procedures.

DATA SELECT PRODUCT CITATIONS

1. Chang, M.C., et al. 2004. The induction of prostaglandin E2 production, interleukin-6 production, cell cycle arrest, and cytotoxicity in primary oral keratinocytes and KB cancer cells by areca nut ingredients is differentially regulated by MEK/ERK activation. J. Biol. Chem. 279: 50676-50683. 2. Beura, L.K., et al. 2011. Cellular poly(c) binding proteins 1 and 2 interact with porcine reproductive and respiratory syndrome virus nonstructural protein 1β and support viral replication. J. Virol. 85: 12939-12949. 3. Guo, X., et al. 2011. The E3 ligase Smurf1 regulates Wolfram syndrome protein stability at the endoplasmic reticulum. J. Biol. Chem. 286: 18037-18047. 4. Xie, P., et al. 2011. Smurf1 ubiquitin ligase targets Krüppel-like factor KLF2 for ubiquitination and degradation in human lung cancer H1299 cells. Biochem. Biophys. Res. Commun. 407: 254-259. 5. Lee, C.C., et al. 2011. Squamocin modulates histone H3 phosphorylation levels and induces G1 phase arrest and apoptosis in cancer cells. BMC Cancer 11: 58. 6. Wei, T.C., et al. 2011. Expression of Crip2, a LIM-domain-only protein, in the mouse cardiovascular system under physiological and pathological conditions. Gene Expr. Patterns 11: 384-394. 7. Ge, Z., et al. 2011. Expression of death decoy receptor-3 (DcR3) in human breast cancer and its functional effects on breast cancer cells in vitro. J. Exp. Ther. Oncol. 9: 109-118. 8. Schuett, H., et al. 2012. Transsignaling of interleukin-6 crucially contributes to atherosclerosis in mice. Arterioscler. Thromb. Vasc. Biol. 32: 281-290. 9.Luo, Y., et al. 2012. Rapamycin inhibits lymphatic endothelial cell tube formation by downregulating vascular endothelial growth factor receptor 3 protein expression. Neoplasia 14: 228-237. 10.Milagre, I., et al. 2012. Neuronal differentiation alters the ratio of Sp transcription factors recruited to the CYP46A1 promoter. J. Neurochem. 120